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6108-17-4
CH3COOLi·2H2O
030601060808010800PD
99%-99.9%
- 200 mesh- 50 mesh
612-081-5
Characteristic
Lithium acetate (CH3COOLi) is a salt of lithium and acetic acid.
Chemical formula:C2H3LiO2
Molar mass: 65.98 g·mol−1
Appearance: crystal
Density: 1.26 g/cm3
Melting point: 286 °C (547 °F; 559 K)
Solubility in water:45.0 g/100 mL
Magnetic susceptibility (χ):−34.0·10−6 cm3/mol
Application
Lithium acetate is used in the laboratory as buffer for gel electrophoresis of DNA and RNA. It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer (5-30V/cm as compared to 5-10V/cm).
Lithium boric acid or sodium boric acid are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA (less than 500 bp) due to the higher resolution of borate-based buffers in this size range as compared to acetate buffers.
Lithium acetate is also used to permeabilize the cell wall of yeast for use in DNA transformation. It is believed that the beneficial effect of LiOAc is caused by its chaotropic effect; denaturing DNA, RNA and proteins.
Characteristic
Lithium acetate (CH3COOLi) is a salt of lithium and acetic acid.
Chemical formula:C2H3LiO2
Molar mass: 65.98 g·mol−1
Appearance: crystal
Density: 1.26 g/cm3
Melting point: 286 °C (547 °F; 559 K)
Solubility in water:45.0 g/100 mL
Magnetic susceptibility (χ):−34.0·10−6 cm3/mol
Application
Lithium acetate is used in the laboratory as buffer for gel electrophoresis of DNA and RNA. It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer (5-30V/cm as compared to 5-10V/cm).
Lithium boric acid or sodium boric acid are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA (less than 500 bp) due to the higher resolution of borate-based buffers in this size range as compared to acetate buffers.
Lithium acetate is also used to permeabilize the cell wall of yeast for use in DNA transformation. It is believed that the beneficial effect of LiOAc is caused by its chaotropic effect; denaturing DNA, RNA and proteins.
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